The purpose of this study is to examine the influence of trainee characteristics towards the effectiveness of transfer of training. The study was conducted in UiTM Shah Alam. Ultimately, this study is aiming to predict the relationship between trainee characteristics and transfer of training. Generally, according to Newstron (1984) and Wexley & Latham (1981), transfer of training can be defined as the degree which an employee who’s going to the training program effectively apply the knowledge, skills and attitudes (KSA) that gained from the program into the job context. The researcher analyzed 176 responses out of 310 by using convenient sampling technique. The primary instrument used was questionnaire whereby the questionnaire has been divided based on three sections which are demographic profile, independent variables and dependent variable. Multivariate analysis of variance using cognitive ability, self-efficacy, motivation and perceived utility of training/instrumentality as independent variables and transfer of training as the dependent variables resulted to have a significant relationship between both variables. It gives prediction to the researcher that employee confidence level on the skills they learned from the training and their abilities to apply the knowledge has derived to the effectiveness of the training in terms of stay up to date on new process, achieve work target, opportunities in different career path and good competitive advantage to the organization for future prospect.