Stress among universities’ students became an issue in today’s world. Stress is undeniable could produce negative implications towards students’ physical and mental health. At worst incident, stress could lead suicidal among students. Therefore, there is an urgent need for the solutions of stress among students. However, the most important part in addressing that issue is to identify the factors that contribute towards students’ stress. Hence, the aims of this research to identify the determinants of stress among private universities students in Selangor specifically from MSU, UniKL and UNISEL. This is because there are not many studies have been done in the private universities’ scope. Secondly, is to examine the moderating effect of gender towards the relationship of independent variables which are academic performance, interpersonal relationships, and financial burdens and dependent variable, namely students’ stress that involved in this study. Lastly is to identify the most influential factor that contributes to students’ stress. In this study, the data obtained from a survey questionnaire that polled students’ information on their stress sources. The data was analyzed and evaluated accordingly to research hypotheses and research objectives. The resulted of this study showed two independent variables in this study which are academic performance and financial burdens are the stressors for students’ stress. Besides, that this research findings also indicates that the most influence factors that contributes towards students’ stress is financial burdens. On the other hand, gender that act as moderating factor moderate the relationship between academic performance and students’ stress only. This study hope that it can contribute towards the understanding in students’ stress factors among private universities’ students in Selangor specifically from MSU, UniKL and UNISEL.