Women have become more social-conscious, they have made progress on their status in family rights and their work life. Women’s roles have enlarged to the point where most of them tend to involve in the workforce and hence, the status of women has increased substantially. In Malaysia banking industry, we can see that nowadays the involvement and the participation from the women are not only in clerical level, but they contribute themselves in the higher levels as well. Secured family life, attractive salary, favourable working conditions and the stability in work are some of the reasons that make this industry more preferable to women. But what actually factors that make them been denied for better career advancement in banking sector? So here, in this study, the focus are more on the relationship between barriers of career advancement among woman in banking sector, where the research study are conducted in Johor Bahru area. Quantitative approach has been used and the data collected throughout the questionnaires survey that has been distributed to the woman in banking industry. Through the finding, the independent variables such as Education Level, Family Consideration as well as Organizational Culture and Structure are significant and positively related to woman career advancement in banking sector compare to other independent variable which are the Work Life Balance and the Glass Ceiling effect. Last but not least, in order to overcome the issues on the barriers on the woman career advancement in banking sector, it is recommended that the government and employers can create a family-friendly environment for working women to overcome the barriers faced by them such as provide childcare support, provide skill training, implement flexible working arrangement, provide employees benefits for woman as well as implement diversity and inclusion policy.