Previous studies suggest that the organizational dynamics of police organizations and the nature of police work contribute to law enforcement stress, which in turn influence job satisfaction as well as effect job performance. It is also well documented that undesirable organizational factors are more hazardous to the well-being of employees than are the stressors due to nature of police work. Using the Police Stress Questionnaire (PSQ) which is the Operational Police Stress Questionnaire (PSQ-Op) and Organizational Police Stress Questionnaire (PSQ-Org) developed by McCreary and Thompson, based on the survey data of 305 Investigation Officers at Royal Malaysia Police (RMP) in Kuala Lumpur Contingent (Response rate = 59.3%), this study examine the factors of psychological stress faced by Investigation Officers at the RMP and to determine the relationship between stress at work and job satisfaction. Using Multiple Regression Analysis where the results of the study contra from previous studies which indicate that Investigation Officers at RMP in Kuala Lumpur Contingent perceived only Operational Stressors have statistically significant negative affects on job satisfaction. Other than that, by using Mean Score Analysis on the survey data indicate that the highest contribution on stress among Investigation Officers at RMP in Kuala Lumpur Contingent is ‘fatigue’. Overall, the findings of this study will assist the RMP top management to understand the stress factors and characteristics that affect job performance. They could then plan and implement assistive programs and procedures for stress management interventions.